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|a Analysis of Diphenhydramine (DPH) in Aqueous Solutions |h [electronic resource]. |
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|a Diphenhydramine (DPH) is an over-the-counter antihistamine which has been reported to be used as a date-rape-drug in drug facilitated crimes (DFC) due to its sedative properties. Due to the high potency and short half-life of DPH, methods that can detect DPH and its metabolites in urine at low concentrations are required. Dispersive liquid-liquid microextraction (DLLME) is a popular extraction technique within the forensic toxicology community due to its low usage of toxic chemicals and speed. DLLME uses a dispersive solvent and an extractive solvent to remove the analyte from the aqueous solution. A DLLME method was used to extract diphenhydramine and one of its metabolites, N-desmethyl diphenhydramine, from aqueous solutions. Aqueous solutions of DPH and its metabolite were prepared in 5 ng/mL to 2 μg/mL ranges. The extractions were conducted at pH of 12 by adding 50 µL of NaOH and 20 µL of phosphate buffer prior to the solution being spiked by 1 mL of a DLLME solvent mixture of toluene and acetonitrile (13:40). After injection, solutions were shaken for 10 min and centrifuged for 10 min. Solutions were then placed into an ice bath for 30 min, and the top toluene layer of each solution was removed and transferred to clean vials. Toluene was then evaporated and the residues were reconstituted with methanol. A second set of solutions used for direct calibration were prepared ranging from 50 ng/mL to 100 µg/mL. The proposed plan was to analyze the extracted compounds using Gas Chromatography coupled with Mass Spectrometry(GC-MS); however, the GC-MS instrument was out of order. Future work includes analysis of the extracted compounds using GC-MS, testing methods to freeze the aqueous layer of the extraction phase using liquid nitrogen, as well as enhancing extraction recoveries of the analytes by conducting a second extraction on the aqueous solutions. |
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|a Electronic reproduction. |c SUNY Oswego Institutional Repository, |d 2020. |f (Oswego Digital Library) |n Mode of access: World Wide Web. |n System requirements: Internet connectivity; Web browser software. |
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|a SUNY Oswego Institution. |
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|a Collected for SUNY Oswego Institutional Repository by the online self-submittal tool. Submitted by Kaylandra Woodside. |
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|a Oswego Digital Library. |
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|a SUNY Oswego Scholarly and Creative Works. |
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|a Summer 2020 Scholarly and Creative Activities Symposium. |
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|a OswegoDL |c SUNY Oswego Scholarly and Creative Works |
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|u https://digitallibrary.oswego.edu/AA00000243/00001 |y Electronic Resource |
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|a https:/digitallibrary.oswego.edu/content/AA/00/00/02/43/00001/K_Woodside_Analysis of Diphenhydramine (DPH)-2 (1)thm.jpg |
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|a SUNY Oswego Scholarly and Creative Works |