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|a The Role of Talin A in KrsB- mediated Adhesion of Dictyostelium discoideum |h [electronic resource]. |
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|a Dictyostelium discoideum serves as an excellent model organism for the study of various
cellular processes, including adhesion. Kinase responsive to stress (KrsB) inhibits adhesion of
cells to the surface; however, the molecular mechanism of KrsB action is unclear. Previous
research has demonstrated that Talin A, an important cytoskeletal protein for cell to substrate
contact in various systems, is involved in basal adhesion of D. discoideum. We hypothesized that
KrsB-mediated effects on adhesion depend on Talin A. To address this question we examined
whether catalytically inactive KrsB (K52R), which is known to increase adhesion, can do so in
the absence of Talin A. We transformed Talin A-null cells with GFP-tagged KrsB K52R or
empty vectors by electroporation and checked cells using fluorescent microscopy to confirm
expression . Following this we completed adhesion assays to compare the two cell lines. There
were no significant differences between adhesion of Talin A-null cells with or without KrsB
RISE 2020 ABSTRACT 2
K52R. Although this preliminary data suggests that Talin A may be required for increased
adhesion in the absence of active KrsB, further research is still needed to confirm these findings. |
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|a Electronic reproduction. |c SUNY Oswego Institutional Repository, |d 2020. |f (Oswego Digital Library) |n Mode of access: World Wide Web. |n System requirements: Internet connectivity; Web browser software. |
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|a SUNY Oswego Institution. |
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|a Collected for SUNY Oswego Institutional Repository by the online self-submittal tool. Submitted by Andrew Nearbin. |
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|a cells adhesion substrate. |
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|a Oswego Digital Library. |
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|a SUNY Oswego Scholarly and Creative Works. |
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|a Summer 2020 Scholarly and Creative Activities Symposium. |
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|a OswegoDL |c SUNY Oswego Scholarly and Creative Works |
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|u https://digitallibrary.oswego.edu/AA00000242/00001 |y Electronic Resource |
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|a SUNY Oswego Scholarly and Creative Works |